Gephyrin antibody - 147 011品牌【Synaptic Systems】

产品名称

Gephyrin antibody

品牌

Synaptic Systems

产品货号

147 011

产品规格

100 µg

产品描述

※Cat. No. 147 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
※Applications
WB: not recommended (see remarks)
IP: not recommended (see remarks)
ICC: 1 : 1000 up to 1 : 2000 gallery
IHC: 1 : 1000 up to 1 : 2000 (see remarks) gallery
IHC-P: not recommended
ExM: yes (see remarks) gallery
DNA-PAINT: yes (see remarks)
Clarity: 1 : 100 (see remarks)
EM: yes
※Clone mAb7a
※Subtype IgG1 (κ light chain)
※Immunogen Nativ Protein corresponding to AA 1 to 768 from rat Gephyrin (UniProt Id: Q03555)
※Epitop Epitop: AA 264 to 276 from rat Gephyrin (UniProt Id: Q03555)
※Reactivity Reacts with: human (Q9NQX3), rat (Q03555), mouse (Q8BUV3), pig, goldfish, zebrafish, chicken.
Other species not tested yet.
※Specificity Specific for the brain specific 93 kDa splice variant phosphorylated at Ser-270. K.O. validated PubMed: 9812897
※Remarks
WB: Clone 3B11 (cat. no. 147 111) is highly recommended.
IP: Clone 3B11 (cat. no. 147 111) is highly recommended.
IHC: Hybridoma supernatant (cat. no. 147 021) highly recommended. For best results use the protocol of Schneider Gasser et al., 2006.
ExM: This antibody has been successfully used for the epitope-preserving magnified analysis of the proteome (eMAP) expansion microscopy method (Park et al. 2021. PMID: 34767453).
DNA-PAINT: This antibody has been successfully used for DNA-PAINT application (see Unterauer et al., 2024; PMID: 38552614).
Clarity: This antibody has been successfully used for CLARITY application in human brain (Woelfle et al., 2023; PMID: 37221592).

保存建议

 Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!