GFAP 173 011 品牌【Synaptic Systems】

Cat. No. 173 011

100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!

Applications

WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: 1 : 2000 up to 1 : 4000 gallery  
Clarity: 1 : 200 video (see remarks)
ELISA: yes (see remarks)

Clone

134B1

Subtype

IgG2a (κ light chain)

Immunogen

full-length recombinant human GFAP (UniProt Id: P14136)

Epitop

Epitop: AA 391 to 405 from human GFAP (UniProt Id: P14136)

Reactivity

Reacts with: human (P14136), rat (P47819), mouse (P03995), cow.
No signal: zebrafish.
Other species not tested yet.

Specificity

Specific for GFAP isoform 1 (alpha) K.O. validated

Matching control protein/peptide

173-0P

Remarks

WB: The monoclonal antibodies are less sensitive compared to the rabbit polyclonal polyclonal (cat. no. 173 002).
Clarity: This antibody has been successfully used for CLARITY application in human brain (Woelfle et al., 2023; PMID: 37221592).
ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 173 002 or 173 211BT as detector antibody.