Gephyrin 147 111 品牌【Synaptic Systems】

Cat. No. 147 111

100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!

Applications

WB: 1 : 1000 up to 1 : 5000 gallery  
IP: yes
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: not recommended
IHC-P: not recommended
IHC-Fr: 1 : 500 (see remarks) gallery  
ELISA: yes

Clone

3B11

Subtype

IgG1 (κ light chain)

Immunogen

Recombinant protein corresponding to AA 307 to 735 from rat Gephyrin (UniProt Id: Q03555)

Epitop

Epitop: AA 326 to 550 from rat Gephyrin (UniProt Id: Q03555)

Reactivity

Reacts with: human (Q9NQX3), rat (Q03555), mouse (Q8BUV3), zebrafish.
Other species not tested yet.

Specificity

Detects all splice variants that contain a complete E-domain including the C6 domain. K.O. validated PubMed: 26829712

Remarks

This antibody is highly recommended for Western blot experiments and immunoprecipitation.
IHC-Fr: Fixation with PFA/formaldehyde or ice-cold methanol/acetone is recommended