Gephyrin 147 021 品牌【Synaptic Systems】

Cat. No. 147 021

300 µl hybridoma supernatant, lyophilized. For reconstitution add 300 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!

Applications

WB: not recommended (see remarks)
IP: not recommended (see remarks)
ICC: 1 : 250 up to 1 : 500 gallery  
IHC: 1 : 100 up to 1 : 250 (see remarks) gallery  
IHC-P: not recommended
ExM: yes
EM: yes

Clone

mAb7a

Subtype

IgG1 (κ light chain)

Immunogen

Nativ Protein corresponding to AA 1 to 768 from rat Gephyrin (UniProt Id: Q03555)

Epitop

Epitop: AA 264 to 276 from rat Gephyrin (UniProt Id: Q03555)

Reactivity

Reacts with: human (Q9NQX3), rat (Q03555), mouse (Q8BUV3), pig, goldfish, zebrafish, frog.
Other species not tested yet.

Specificity

Specific for the brain specific 93 kDa splice variant phosphorylated at Ser-270. K.O. validated

Remarks

WB: Clone 3B11 (cat. no. 147 111) is highly recommended.
IP: Clone 3B11 (cat. no. 147 111) highly recommended.
IHC: Hybridoma supernatant highly recommended. For best results use the protocol of Schneider Gasser et al., 2006.
Alternatively antigen retrieval (10 mM citrate, pH 6.0, over night at 60°C) can be applied.

Glyoxal fixation according to Konno et al. 2023 has improved signal quality and strength.