Gephyrin 147 008 品牌【Synaptic Systems】

Cat. No. 147 008

50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!

Applications

WB: yes (see remarks)
IP: yes (see remarks)
ICC: 1 : 500 gallery  
IHC: 1 : 500 up to 1 : 1000 (see remarks) gallery  
IHC-P: not recommended
ExM: yes (see remarks) gallery  

Clone

RbmAb7a

Subtype

IgG1 (κ light chain)

Immunogen

Nativ Protein corresponding to AA 1 to 768 from rat Gephyrin (UniProt Id: Q03555)

Epitop

Epitop: AA 264 to 276 from rat Gephyrin (UniProt Id: Q03555)

Reactivity

Reacts with: human (Q9NQX3), rat (Q03555), mouse (Q8BUV3), pig, goldfish, zebrafish.
Other species not tested yet.

Specificity

Specific for the brain specific 93 kDa splice variant phosphorylated at Ser-270. K.O. validated

Remarks

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody mAb7a. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. The antibody can therefore be used with standard anti-rabbit secondary reagents.
The antibody has been expressed in mammalian cells.
WB: Clone 3B11 (cat. no. 147 111) is highly recommended.
IP: Clone 3B11 (cat. no. 147 111) is highly recommended.
IHC: For best results use the protocol of Schneider Gasser et al., 2006.
Alternatively antigen retrieval (10 mM citrate, pH 6.0, over night at 60°C) can be applied.
ExM: This antibody has been successfully used for the epitope-preserving magnified analysis of the proteome (eMAP) expansion microscopy method (Park et al. 2021. PMID: 34767453).