anti-DNA, AC-30-10, mouse mab 690014S 品牌【Progen】
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Immunofluorescence analysis of HeLa cells with anti-DNA antibody. Fixation was performed using 3% paraformaldehyde for 10 min at RT. Cells were blocked with 5% BSA in PBST (PBS + 0.1% Tween 20) for 1 h at RT and permeabilized with 0.3% Triton-X 100 in PBS for 10 min at RT. The primary antibody anti-DNA mouse monoclonal, AC-30-10 (Cat. No. 690014) was diluted in blocking buffer (antibody concentration 0.5 µg/ml) and incubated over-night at 4°C. The secondary antibody goat anti-mouse IgM Alexa488 was also diluted in blocking buffer (antibody concentration 2.5 µg/ml) and incubated for 30 min at 37°C and 30 min at RT. Nuclear DNA was stained with DAPI in blue.
PRODUCT DESCRIPTION
Quantity
200 µl
Antibody Type
Monoclonal
Host
Mouse
Isotype
IgM
Conjugate
Unconjugated
Application
Dot blot, ICC/IF, IHC
Purification
Size exclusion chromatography
Reactivity
All species
Storage
2-8°C
Intended use
Research use only
Clone
AC-30-10
Immunogen
Double- and single-stranded DNA
Concentration
100 µg/ml (20 µg)
Formulation
PBS pH 7.4 with 0.09% sodium azide and 0.5% BSA
APPLICATIONS
Tested applications
Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF)
1:200 (0.5 µg/ml)
Immunohistochemistry (IHC) - frozen
1:10 (10 µg/ml)
Immunohistochemistry (IHC) - paraffin
1:10 (10 µg/ml, microwave treatment recommended)
Dot Blot
Assay dependent (on nitrocellulose membrane, after baking at 70°C)
