AAV2 empty capsids 66V020 品牌【Progen】
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PRODUCT DESCRIPTION
Quantity
100 µl (> 5.0E+11 capsids)
Application
Dot blot, ELISA, WB
Purification
Affinity Chromatography (POROS CaptureSelect AAVX Affinity Resin, Thermo Fisher Scientific)
Storage
Up to 2 weeks: 2-8°C; long term storage in aliquots at -80°C; avoid > 5 freeze/thaw cycles
Intended use
Research use only
Concentration
> 5.0E+12 capsids/ml; please find the lot-specific concentration on the CoA and on the vial
Formulation
PBS + 0.014% Tween20 + 1 mM MgCl2 + 2.5 mM KCl
Source
Produced in HEK293T cells
Purity
> 95% by SDS-PAGE and silver stain
Quality check
Final titer was assigned on internal reference material using two AAV2 ELISA kits (PRAAV2R and PRAAV2XP); QC included analysis of filling grade and Endotoxin testing
Packaging Plasmid
pDP2rs
Product description
Purified empty adeno-associated virus 2 capsids (AAV2)
Endotoxin
< 1.0 EU/ml
Note
Please centrifuge before opening to ensure complete recovery of vial contents; repeated freeze/thaw cycles and aliquoting can lead to a drop in titer
APPLICATIONS
Tested applications
Tested dilutions
Dot Blot
Depending on primary antibody and detection method
ELISA
As a positive control in ELISA, dilute in ASSB 1x (provided with PROGEN’s AAV2 ELISA) and analysis at least in duplicates is recommended
Western Blot (WB)
Depending on primary antibody and detection method

