AAV2 empty capsids 66V020 品牌【Progen】

Quantity

100 µl (> 5.0E+11 capsids)

Application

Dot blot, ELISA, WB

Purification

Affinity Chromatography (POROS CaptureSelect AAVX Affinity Resin, Thermo Fisher Scientific)

Storage

Up to 2 weeks: 2-8°C; long term storage in aliquots at -80°C; avoid > 5 freeze/thaw cycles

Intended use

Research use only

Concentration

> 5.0E+12 capsids/ml; please find the lot-specific concentration on the CoA and on the vial

Formulation

PBS + 0.014% Tween20 + 1 mM MgCl2 + 2.5 mM KCl

Source

Produced in HEK293T cells

Purity

> 95% by SDS-PAGE and silver stain

Quality check

Final titer was assigned on internal reference material using two AAV2 ELISA kits (PRAAV2R and PRAAV2XP); QC included analysis of filling grade and Endotoxin testing

Packaging Plasmid

pDP2rs

Product description

Purified empty adeno-associated virus 2 capsids (AAV2)

Endotoxin

< 1.0 EU/ml

Note

Please centrifuge before opening to ensure complete recovery of vial contents; repeated freeze/thaw cycles and aliquoting can lead to a drop in titer

Tested applications

Tested dilutions

Dot Blot

Depending on primary antibody and detection method

ELISA

As a positive control in ELISA, dilute in ASSB 1x (provided with PROGEN’s AAV2 ELISA) and analysis at least in duplicates is recommended

Western Blot (WB)

Depending on primary antibody and detection method