VGLUT 2 -135402 品牌【Synaptic Systems】

Cat. No. 135 402

200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!

Applications

WB: 1 : 1000 up to 1 : 10000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 250 up to 1 : 1000 gallery  
IHC-P: 1 : 500 gallery  
ExM: 1 : 250 (see remarks) gallery  

Immunogen

Synthetic peptide corresponding to residues near the carboxy terminus of rat VGLUT2 (UniProt Id: Q9JI12)

Reactivity

Reacts with: human (Q9P2U8), rat (Q9JI12), mouse (Q8BLE7), chicken.
Other species not tested yet.

Matching control protein/peptide

135-4P

Remarks

This antibody is highly recommended as a marker for glutamatergic nerve terminals.
WB: VGLUT 2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
ExM: This antibody has been successfully used for the magnified analysis of the proteome (MAP) expansion microscopy method (MAP; Ku et al. 2016. Nature Biotechnology 34:973-981)