VGAT antibody cytoplasmic domain-131003 品牌【Synaptic Systems】

产品名称

VGAT antibody cytoplasmic domain

品牌

Synaptic Systems

产品货号

131 003

产品规格

50 µg

产品描述

※Cat. No. 131 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
※Applications
WB: 1 : 1000 up to 1 : 5000 (AP staining) (see remarks) gallery
IP: yes
ICC: 1 : 100 up to 1 : 1000 gallery
IHC: 1 : 500 up to 1 : 3000 gallery
IHC-P: 1 : 200 up to 1 : 500 gallery
ExM: yes (see remarks) gallery
EM: yes
※Immunogen Synthetic peptide corresponding to residues near the amino terminus of rat VGAT (UniProt Id: O35458)
※Reactivity Reacts with: human (Q9H598), rat (O35458), mouse (O35633), monkey.
Other species not tested yet.
※Specificity K.O. validated PubMed: 19052203
※Matching control protein/peptide 131-0P
※Remarks
WB: VGAT aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
ExM: This antibody has been successfully used for the epitope-preserving magnified analysis of the proteome (eMAP) expansion microscopy method (Park et al. 2021. PMID: 34767453).

保存建议

Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!