Synaptobrevin 2-104008 品牌【Synaptic Systems】

Cat. No. 104 008

50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!

Applications

WB: yes AP staining gallery  
IP: yes (see remarks)
ICC: 1 : 500 up to 1 : 2000 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 5000 gallery  

Clone

Rb69.1

Subtype

IgG1 (κ light chain)

Immunogen

Synthetic peptide corresponding to residues near the amino terminus of rat Synaptobrevin2 (UniProt Id: P63045)

Reactivity

Reacts with: mouse (P63044), rat (P63045), human (P63027), hamster.
No signal: chicken, zebrafish.
Other species not tested yet.

Specificity

K.O. validated

Matching control protein/peptide

104-2P

Remarks

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody 69.1. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
IP: This antibody quantitatively precipitates synaptobrevin 2 from detergent extracts regardless of whether the protein is associated.