PSD95 N3783 品牌【Synaptic Systems】

This product was developed by   

Cat. No. N3783

100 µg purified antibody lyophilized from PBS. Reconstitute with 100 µL 50% glycerol and store at -20°C or below.

Applications

WB: 1 : 1000 gallery  
IP: not tested yet
ICC: 1 : 500 (see remarks) gallery  
IHC: 1 : 200 up to 1 : 500 (see remarks) gallery  
IHC-P: 1 : 200 gallery  
ExM: yes (see remarks) gallery  

Clone

1B2

Subtype

IgG1

Immunogen

Recombinant protein corresponding to AA 68 to 251 from mouse PSD95 (UniProt Id: Q62108)

Reactivity

Reacts with: mouse (Q62108), rat (P31016), human (P78352).
Other species not tested yet.

Specificity

K.O. validated

Matching control protein/peptide

124-01P

Remarks

This antibody is a chimeric construct consisting of the anti-PSD 95 single-domain antibody 1B2 genetically fused to rabbit IgG1 Fc domain. It can be used in combination with standard anti-rabbit secondary reagents binding the IgG Fc-domain.
ICC: We recommend the PSD95 FluoTags N3702 for best results. 
IHC: We recommend the PSD95 FluoTags N3702 for best results. 
ExM: This antibody has been successfully used for the epitope-preserving magnified analysis of the proteome (eMAP) expansion microscopy method (Park et al. 2021. PMID: 34767453).
Variance from the standard protocol:
Clearing after gel-embedding: overnight at 37°C followed by 10 minutes at 88°C.