MAP 2 188 011 品牌【Synaptic Systems】

Cat. No. 188 011

100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!

Applications

WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 100 up to 1 : 500 gallery  
IHC: 1 : 100 up to 1 : 200 gallery  
IHC-P: 1 : 500 gallery  
DNA-PAINT: yes (see remarks)

Clone

198A5

Subtype

IgG1 (κ light chain)

Immunogen

Recombinant protein corresponding to residues near the amino terminus of human Map2 (UniProt Id: P11137-4)

Epitop

Epitop: AA 82 to 96 from human MAP2-4 hu (UniProt Id: P11137-4)

Reactivity

Reacts with: human (P11137), rat (P15146), mouse (P20357).
No signal: zebrafish.
Other species not tested yet.

Specificity

Specific for MAP 2; recognizes all four isoforms.

Matching control protein/peptide

188-0P

Remarks

WB: Due to its large size, MAP 2 requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.
DNA-PAINT: This antibody has been successfully used for DNA-PAINT application (see Unterauer et al., 2024; PMID: 38552614).