GluN 1 114 011 品牌【Synaptic Systems】

Cat. No. 114 011

100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!

Applications

WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery  
IP: yes (see remarks)
ICC: 1 : 1000 (see remarks) gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P: 1 : 1000 (see remarks) gallery  
IHC-G: (see remarks) gallery  
ExM: 1 : 250 (see remarks) gallery  
ELISA: yes (see remarks)

Clone

M68

Subtype

IgG2b (κ light chain)

Immunogen

Recombinant protein corresponding to AA 660 to 811 from rat GluN1 (UniProt Id: P35439)

Reactivity

Reacts with: human (Q05586), rat (P35439), mouse (P35438), zebrafish.
Other species not tested yet.

Specificity

K.O. validated PubMed: 24344200

Remarks

This antibody is suitable for the surface staining of living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondary reagents.
IP: For most effective IP, use the denaturing IP-protocol. Consider that protein-protein interactions may be affected.
ICC: Methanol fixation is recommended.
IHC: Heat mediated antigen retrieval (AGR) at pH 6,0 is recommended.
IHC-P: Requires heat mediated antigen retrieval at pH 9.0 for 30min at 97°C.
IHC-G: Fixation with 9% glyoxal, 8% acetic acid, in ddH2O, pH 4.2-4.4, according to Konno et al. 2023 is recommended.
ExM: This antibody has been successfully used for the magnified analysis of the proteome (MAP) expansion microscopy method (MAP; Ku et al. 2016. Nature Biotechnology 34:973-981).
This antibody has been successfully used for the epitope-preserving magnified analysis of the proteome (eMAP) expansion microscopy method (Park et al. 2021. PMID: 34767453).
ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 114 003 as detector antibody. The ELISA-protocol for membrane proteins is recommended.