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ALFA sdAb N1502-AF647-L 品牌【Synaptic Systems】

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    ALFA sdAb - FluoTag-X2 - N1502-AF647-L

    The ALFA tag is a novel peptide tag

     

     

    Camelid single domain antibodies consist only of one antigen binding site of a Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules. FluoTags carry fluorophore molecules coupled in a site specific manner.

     

    The ALFA-tag is a novel, rationally designed epitope tag (SRLEEELRRRLTE) that forms a small and stable α-helix that is functional irrespective of its position on the target protein in prokaryotic and eukaryotic hosts.

    Camelid single domain antibody

    Cat. No. N1502-AF647-L

    200 µl purified antibody, lyophilized from PBS, fluorescence-labeled with Alexa 647. Albumin was added for stabilization. For reconstitution add 200 µl H2O. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
    Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.

    Storage

    Up to three months: -20°C
    Up to 12 months: -80°C or below
    Protect from light!

    Applications

     

    ICC: 1 : 500
    FACS: yes

    Label

    Alexa 647, two fluorophores coupled to one FluoTag

    Clone

    1G5

    Subtype

    single domain

    Immunogen

    Synthetic peptide corresponding to: SRLEEELRRRLTE (UniProt Id: not defined)

    Specificity

    Recognizes the ALFA tag: SRLEEELRRRLTE

  • 品牌
    Synaptic Systems