VGLUT 1 135 304 品牌【Synaptic Systems】

Cat. No. 135 304

100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!

Applications

WB: 1 : 5000 (AP staining) gallery  
IP: yes
ICC: 1 : 1000 up to 1 : 5000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: 1 : 200 gallery  
IHC-Fr: 1 : 750 (see remarks)
ExM: 1 : 1000
Clarity: 1 : 200 (see remarks)
EM: yes
FACS: yes

Immunogen

Recombinant protein corresponding to residues near the carboxy terminus of rat VGLUT 1 (UniProt Id: Q62634)

Reactivity

Reacts with: rat (Q62634), mouse (Q3TXX4), human (Q9P2U7), cow.
Other species not tested yet.

Specificity

K.O. validated PubMed: 34876472

Matching control protein/peptide

135-3P

Remarks

VGLUT 1 aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples.
IHC-Fr: 5 min MeOH and PFA fixation are possible.
Clarity: This antibody has been successfully used for CLARITY application in human brain (Woelfle et al., 2023; PMID: 37221592).