弹性蛋白酶 LS002292 品牌【Worthington】

产品名称

弹性蛋白酶【Elastase】

品牌

Worthington

产品货号

LS002292

产品规格

100 mg

产品描述

Porcine pancreatic elastase has a molecular weight of 25.9 kDa, and a pH optimum of 8.5. While elastase will hydrolyze a wide variety of protein substrates, it is unique among proteases in its ability to hydrolyze native elastin, a substrate not attacked by trypsin, chymotrypsin or pepsin. Soybean trypsin inhibitor and kallikrein inhibitor suppress proteolytic but not elastolytic activity. Elastase is assayed using a method adapted from that of Feinstein et al., Biochem. Biophys. Res. Comm., 50, 1020 (1973) and using the more soluble substrate of Bieth et al., Biochem. Med., 11, 350 (1974).

应用场景

组织解离，单细胞制备

保存建议

Two times crystallized, (Code: ESL), supplied as a dialyzed, lyophilized powder. The enzyme should be 0.22 micron filtered after reconstitution and prior to use. Suitable for the isolation of Type II lung cells.
Store at 2-8°C.