VGLUT抗体 - 135 303 品牌【Synaptic Systems】

产品名称

VGLUT抗体【VGLUT1 antibody】

品牌

Synaptic Systems

产品货号

135 303

产品规格

50 µg

产品描述

※Cat. No. 135 303 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
※Applications
WB: 1 : 1000 up to 1 : 10000 (AP staining) (see remarks) gallery
IP: yes
ICC: 1 : 500 up to 1 : 10000 gallery
IHC: 1 : 1000 gallery
IHC-P: 1 : 500 gallery
IHC-Fr: 1 : 500 (see remarks)
DNA-PAINT: yes (see remarks)
Clarity: yes
EM: yes
ELISA: yes (see remarks)
※Immunogen Recombinant protein corresponding to residues near the carboxy terminus of rat VGLUT 1 (UniProt Id: Q62634)
※Reactivity Reacts with: human (Q9P2U7), rat (Q62634), mouse (Q3TXX4), cow, goat, sheep, dog.
Other species not tested yet.
※Specificity K.O. validated PubMed: 15103023
※Matching control protein/peptide 135-3P
※Remarks
This antibody is highly recommended as marker for glutamatergic nerve terminals.
WB: VGLUT 1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
IHC-Fr: 5 min MeOH and PFA fixation are possible.
DNA-PAINT: This antibody has been successfully used for DNA-PAINT application (see Unterauer et al., 2024; PMID: 38552614).
ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 135 311 as capture antibody. The ELISA-protocol for membrane proteins is recommended.

应用场景

抗体

保存建议

Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!