Synaptophysin抗体 - 101 011 品牌【Synaptic Systems】

产品名称

Synaptophysin抗体【Synaptophysin1 antibody】

品牌

Synaptic Systems

产品货号

101 011

产品规格

50 µg

产品描述

※Cat. No. 101 011 50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
※Applications
WB: 1 : 10000 (AP staining) gallery
IP: yes
ICC: 1 : 100 up to 1 : 1000 gallery
IHC: 1 : 500 up to 1 : 1000 gallery
IHC-P: 1 : 500 up to 1 : 1000 gallery
ExM: yes (see remarks) gallery
EM: yes
ELISA: yes (see remarks)
※Clone 7.2
※Subtype IgG1 (λ light chain)
※Immunogen Full-length recombinant rat Synaptophysin (UniProt Id: P07825)
※Epitop Epitop: AA 219 to 307 from rat Synaptophysin1 (UniProt Id: P07825)
corresponding to the cytoplasmic tail.
※Reactivity Reacts with: human (P08247), rat (P07825), mouse (Q62277), mammals.
Weaker signal: zebrafish, other vertebrates.
Other species not tested yet.
※Specificity K.O. validated PubMed: 31940485
※Remarks
Widely used as marker for nerve terminals and neuroendocrine tumors. For unknown reason, neuronal synaptophysin is better recognised than neuroendocrine synaptophysin. If this is a problem, the polyclonal rabbit antibody, cat. no. 101 002 or 101 203 are recommended.
ExM: This antibody has been successfully used for the epitope-preserving magnified analysis of the proteome (eMAP) expansion microscopy method (Park et al. 2021. PMID: 34767453).
Variance from the standard protocol:
Expansion solution for imaging: 0.02X PBS
ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 101 002 as detector antibody. The ELISA-protocol for membrane proteins is recommended.

应用场景

抗体

保存建议

Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!