全新DNA甲基化（5-mc）极速分析试剂盒（比色法）P-1030 品牌【Epigentek】

产品名称

全新DNA甲基化（5-mc）极速分析试剂盒（比色法）【
MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric)】

品牌

Epigentek

产品货号

P-1030

产品规格

48 reactions/96 reactions

产品描述

The MethylFlash™ Global DNA Methylation (5-mC) ELISA Easy Kit is a complete set of optimized buffers and reagents to colorimetrically quantify global DNA methylation status by specifically measuring levels of 5-methylcytosine (5-mC) in a simplified, "one-step" ELISA-like reaction. This allows global DNA methylation to be directly measured more quickly than other techniques such as LUMA, LINE-1, Alu or LTR-based assays. As a fourth generation technology of EpigenTek's patented global DNA methylation technique, it is a further refinement of the popular, predecessor MethylFlash kit by improving upon speed, simplicity, sensitivity, and reproducibility.
This kit is also specifically optimized for paired use with the MethylFlash Global DNA Hydroxymethylation (5-hmC) ELISA Easy Kit (Colorimetric) for simultaneously quantifying both methylated DNA and hydroxymethylated DNA.

This kit has the following advantages and features:

Fast - Reduced steps so that the entire procedure only needs 2 hours
Robust - Improved kit composition allows the assay to have a greater "signal window" with reduced variation between replicates
Convenient - Inherently low background noise, thereby eliminating the need for DNA denaturation and plate blocking steps
Sensitive - Detection limit can be as low as 0.05% methylated DNA from 100 ng of input DNA
Specific - High specificity to 5-mC, with no cross-reactivity to unmethylated cytosine and no cross-reactivity to hydroxymethylcytosine within the indicated concentration range of the sample DNA
Universal - Positive and negative controls are included and allow for detection of DNA methylation in any species from either single-stranded or double-stranded input DNA
Accurate - Optimized positive controls that can be fractionalized in percentage scale, allowing the assay to be more accurate and highly comparable with HPLC-MS analysis
Flexible - Strip-well microplate format makes the assay available for manual or high throughput analysis

应用场景

DNA甲基化程度定量

保存建议

在接收到Epigentek试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分