PROTEOSTAT®蛋白聚集体检测试剂盒 ENZ-51035-K100 品牌【Enzo】

产品名称

PROTEOSTAT®蛋白聚集体检测试剂盒 【PROTEOSTAT® Aggresome detection kit】

品牌

Enzo

产品货号

ENZ-51035-K100

产品规格

100 tests

产品描述

Robust, quantitative detection of aggresomes relevant to the study of neurodegenerative disease, liver disease and toxicology

 Provides a sensitive cell-based assay of drug responsiveness to identify inhibitors relevant to neurodegenerative disease in an authentic cellular context
 Reliable and simple assay doesn’t require non-physiological protein mutations or genetically engineered cell lines
 Validated under a wide range of conditions and with small molecule modulators demonstrating suitability for screening compounds of potential therapeutic value
 Fixed-cell assay is optimized for antibody co-localization studies
 Easily quantifies aggresome and related inclusion bodies by flow cytometry
 Useful for the study of neurodegenerative diseases, liver disease, toxicology studies and much more
Aggresomes are inclusion bodies of aggregated, misfolded proteins that form when the ubiquitin-proteasome protein-degradation machinery is overwhelmed. Typically, aggresomes form in response to cellular stress and provide a cytoprotective mechanism by isolating misfolded proteins, leading ultimately to their clearance by autophagy. Protein aggregate formation is furthermore a hallmark of a variety of human diseases, such as Alzheimer’s, Parkinson’s, Amyotrophic lateral sclerosis or alcoholic liver disease.

The PROTEOSTAT® Aggresome Detection Reagent contained in this kit is a molecular rotor dye. While in solution, free intramolecular rotation along a single central bond prevents fluorescence. The PROTEOSTAT® dye specifically intercalates into the cross-beta spine of quaternary protein structures typically found in misfolded and aggregated proteins, which will inhibit the dye’s rotation and lead to a strong fluorescence.

PROTEOSTAT® Aggresome Detection kit provides a rapid, specific and quantitative approach to label aggresomes in cells, eliminating the need of artificial protein mutations. PROTEOSTAT® dye has been validated under a wide range of conditions and with small molecule modulators, demonstrating its suitability for compound screening. Furthermore, it is suitable for multiplex co-immunofluorescence to study your target of interest in the context of autophagy and aggresome formation.
 

应用场景

生物工艺

保存建议

短期储存-20℃，长期储存-80℃